iX-Pure™ Rapid DyeTerminator cleanup

The iX-Pure™ purification kit is a fast purification method

for DNA sequencing reactions that removes unincorporated

DyeTerminators

and salts. The cleanup is complete in under

40 minutes and requires minimal hands-on time.

Compatibility

These reagents can be added as a premix or sequentially.

Compatible

with BigDye XTerminator run modules.

It is essential to remove the contaminants, especially the

unincorporated

ddNTP dyes, from the completed sequencing

reaction. Failure to remove these contaminants results in signal

reduction and interference of peak separation and reading.

Different mechanisms are available for cleaning up the

sequencing

reactions. In one mechanism, contaminants bind to

special resins and get removed. In another mechanism, extension

products bind to magnetic beads and are separated from

contaminants. Contaminants can also be removed by ethanol

precipitation of DNA, a time-consuming procedure. It is

important to compare the performance of different purification

kits in terms of downstream sequencing quality.

We provide iX-Pure™ Purification Kit and iX-Pure™ Sequencing

Reaction Cleaning Beads, both of which are optimized for

downstream sequencing performance